Processing semen

 

Introduction

AI lab layout

Entering the laboratory

Routine semen diluent BTS

Semen processing

Semen processing pictures

Semen Tests

Dispensing semen

Dispensing pictures

After Processing

Laboratory Checks

Common errors

Semen morphology staining

Semen morphology

 

Tests on the semen system

 

Boar health

Ejaculation details

Routine records

Colorimeter

Semen morphology

Daily checks

Daily checks

AI storage controls

 

Inspections:

Manager

Veterinarian

 

 

Notes on Semen Tests

 

1

 

Semen motility is best assessed by video on the microscope then any questionable boar can be examined and discussed with the lab team/manager

2

Semen motility can be seriously affected by cold microscope slides or if examined after 30 seconds on the slide unless a heated microscope stage is used

3

Many labs try to examine individual sperm at 400x. However, this is generally unrewarding.  Remember it is the population of sperm health that matters.

4

There is no need to use a coverslip, this only increases processing time and increases health risks from broken glass and disposal.

5

Ensure that the drive for perfection is realistic. None of the current simple or more complex tests within the costs of a commercial or farm lab have any bearing on fertility excluding dead or alive assessments and even then many cases of poor boars are actually man made in the lab and have no true bearing on the boar health/productivity

6

There is currently no relationship between motility and fertility if the motility score is over 60% or a motility score of 2 depending on scale used

 

If you have a doubtful boar always check your own procedures first rather than assume the worse for the boar