Processing semen

Introduction	AI lab layout	Entering the laboratory
Routine semen diluent BTS	Semen processing	Semen processing pictures
Semen Tests	Dispensing semen	Dispensing pictures
After Processing	Laboratory Checks	Common errors
Semen morphology staining		Semen morphology

AI Laboratory entry procedures

This includes the semen processing and storage area

On entry into the laboratory changing room
1	Wash your hands using hand disinfectant before entering laboratory changing room
2	Dip wellingtons into foot bath outside laboratory containing disinfectant. Remove all straw on unit overalls If wellingtons are very dirty, clean them and then re-wash hands carefully removing all traces of disinfectant. If overalls are very dirty, go and change into clean unit overalls. Yard Boots and overalls must not enter the laboratory changing area
2	When entering changing room, remove unit overalls and wellingtons
3	Step over barrier
4	Change into laboratory white overalls and wellingtons

On entry into the laboratory
1	On entry into the laboratory wash your hands using a hand disinfectant immediately
2	Switch on the water bath, set at 35^oC, warming plate 35^oC and microscope. Colorimeter if used. These may take 30 minutes to warm up
3	Check the warming incubator is at 37^oC (if used) and the semen storage cabinet/room is at 17^oC
4	Wipe down all working surfaces with a clean damp cloth with hand disinfectant as the soap
5	Prepare the semen diluent