Tests on the semen system

 

Boar health

Ejaculation details

Routine records

Colorimeter

Semen morphology

Daily checks

Daily checks

AI storage controls

 

Inspections:

Manager

Veterinarian

 

 

AI sTorage controls

Daily checks on stored diluted semen

 

Without caffeine ideal but more time needed

 

1

Check the motility of each test bottle (T) prepared today

2

Check the motility of each stored test bottles (T) from other days

3

It is imperative to warm the sample to 35C for at least 30 minutes before evaluation

4

Evaluate the motility at x200 magnification and record the result

 

 

With caffeine

 

1

Using Caffeine coated slides, add a drop of semen to the slide or

 

Mix 1 ml of semen with 1 ml of 0.1% caffeine solution

3

It is imperative to warm the sample to 35C for one minute before evaluation. It is not necessary to warm stored samples

4

Evaluate the motility at x200 magnification and record the result

 

 

Note there are several types of semen extenders which contain ingredients that suppress the motility of the sperm even under ideal conditions. To gain any idea of diluted semen motility in the laboratory it may be necessary to use caffeine coated slides.

 

Typical extenders which suppress sperm motility are Andrahep or Modex.